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Updated: Sep 4, 2025

Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
Published on: January 12, 2015
The disconnect2 mutation disrupts the tjp1b gene that is required for electrical synapse formation
Jennifer Carlisle Michel1, Abagael M Lasseigne1, Audrey J Marsh1
1Institute of Neuroscience, University of Oregon, Eugene, OR USA.
Abstract:
To investigate electrical synapse formation in vivo we used forward genetics to disrupt genes affecting Mauthner cell electrical synapses in larval zebrafish. We identify the disconnect2 ( dis2 ) mutation for its failure to localize neural gap junction channels at electrical synapses. We mapped this mutation to chromosome 25 and identified a splice-altering mutation in the tjp1b gene. We demonstrated that the dis2 mutation disrupts tjp1b function using complementation analysis with CRISPR generated mutants. We conclude that the dis2 mutation disrupts the tjp1b gene that is required for electrical synapse formation.
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