Boosting hydrogen production from fermentation effluent of biomass wastes in cylindrical single-chamber microbial...

Microbial electrolysis cells (MECs) are considered as green technologies for H₂ production with simultaneously wastewater treatment. Low H₂ recovery and production rate are two key bottlenecks of MECs fed with real H₂ fermentation effluent of biomass wastes. This work established a 1 L cylindrical single chamber MEC and enriched electroactive anodic biofilms from cow dung compost to overcome the bottlenecks. These MEC components (platinum-coated cylindrical titanium mesh cathode and cylindrical graphite felt anode) were arranged in a concentric configuration. A high H₂ production rate of 6.26 ± 0.23 L L^-1 day^-1 with H₂ yield of 5.75 ± 0.16 L H₂ L^-1 fermentation effluent was achieved at 0.8 V. The degradation of acetate and butyrate (main components of H₂ fermentation effluent) could reach to 95.3 ± 2.1% and 78.4 ± 3.6%, respectively. The microbial community analysis for anode showed the abundance of Geobacter (22.6%), Syntrophomonas (8.7%), and Dysgonomonas (6.3%) which are responsible to complex substrate oxidation, electrical current generation, and H₂ production. These results prove the feasibility of cylindrical single-chamber MEC for high H₂ production rate and high acetate and butyrate removal from H₂ fermentation effluent.