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Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Hypoglycemia and Glucagon

Hypoglycemia and Glucagon

Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...

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Related Experiment Video

Updated: Sep 4, 2025

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Explaining unexplained hypoglycemia: How LC-MS/MS can help.

M T Ackermans¹, J Hopman², A C Heijboer^1,3

¹Endocrine Laboratory, Department of Clinical Chemistry, Amsterdam UMC, University of Amsterdam, the Netherlands.

Practical Laboratory Medicine

|July 21, 2022

Summary

This summary is machine-generated.

Diagnosing unexplained hypoglycemia is difficult. Liquid chromatography-mass spectrometry (LC-MS/MS) can identify specific insulin analogs, aiding clinical diagnosis and patient discussions.

Area of Science:

Clinical Chemistry
Analytical Chemistry
Endocrinology

Background:

Hypoglycemia in non-diabetic patients presents diagnostic challenges.

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Accurate insulin level determination is crucial for diagnosing endogenous hyperinsulinism.

Distinguishing between endogenous insulin and insulin analogs is often necessary.