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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Related Experiment Video

Updated: Sep 4, 2025

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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Structures of multisubunit membrane complexes with the CRYO ARM 200.

Christoph Gerle1,2, Jun-Ichi Kishikawa1, Tomoko Yamaguchi3

  • 1Institute for Protein Research, Osaka University, 3-2 Yamada Oka, Suita, Osaka 565-0871, Japan.

Microscopy (Oxford, England)
|July 21, 2022
PubMed
Summary

The JEOL CRYO ARM 200 prototype enables atomic model building of membrane protein complexes using single-particle analysis. This cryo-electron microscopy (cryo-EM) method advances structural membrane biology.

Keywords:
PSIV-ATPasemolecular motorprotein structuretransmission electron microscope

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Area of Science:

  • Structural biology
  • Membrane protein complex analysis
  • Cryo-electron microscopy (cryo-EM)

Background:

  • The 'Resolution Revolution' in cryo-EM has accelerated structural membrane biology.
  • Single-particle analysis (SPA) is key for atomic model building of multisubunit membrane proteins.
  • Increased demand for cryo-EM necessitates new, affordable microscope technologies.

Purpose of the Study:

  • To evaluate the JEOL CRYO ARM 200 prototype for SPA.
  • To determine the structures of three distinct multisubunit membrane complexes.

Main Methods:

  • Single-particle analysis (SPA) using cryo-electron microscopy (cryo-EM).
  • Utilized the JEOL CRYO ARM 200 prototype microscope.
  • Focused on three specific membrane protein complexes.

Main Results:

  • Successful structure determination of the Thermus thermophilus V-type ATPase VO complex.
  • Determined the structure of the Thermosynechococcus elongatus photosystem I monomer.
  • Resolved the structure of the Salmonella enterica flagellar motor lipopolysaccharide peptidoglycan ring (LP ring).

Conclusions:

  • The JEOL CRYO ARM 200 prototype is effective for SPA.
  • This technology aids in determining atomic models of complex membrane proteins.
  • Advances in cryo-EM instrumentation support structural membrane biology research.