Selective and sensitive fluorescence turn-on detection of bilirubin using resorcinol-sucrose derived carbon dot

Carbon dots have been prepared from resorcinol and sucrose (rsCDs) hydrothermally, which show green emission at 525 nm with a fluorescence quantum yield (PLQY) of 17.2%. The intense emission of rsCDs is quenched upon the addition of Cu²⁺. In the presence of bilirubin (BR), the emission intensity is enhanced due to the competitive binding of Cu²⁺ with bilirubin and hence releasing rsCDs to the sensing medium. It is the first time report on turn-on fluorescence sensing towards BR with a detection limit of 85 nM. Even in the presence of other comparable biomolecules, the sensor is selective and ultrasensitive to bilirubin. A cellulose paper-based sensor strip has also been designed for the naked-eye detection of BR in blood serum. Due to the specific recognition of this rsCDs towards BR, it can be applied to detect BR in practical human serum samples.