Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

56.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.7K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.4K
5.4K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.6K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.7K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.7K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

65.0K
65.0K
RNA Editing02:23

RNA Editing

9.1K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcript-Level Modulation of O-GlcNAc Transferase for Aging-Related Neurodegenerative Diseases.

Chembiochem : a European journal of chemical biology·2026
Same author

Helical aromatic oligoamide foldamers as selective G-quadruplex ligands.

Nucleic acids research·2025
Same author

From paleness to albinism: Contribution of OCA2 exon 10 skipping to hypopigmentation.

PLoS genetics·2025
Same author

Functional Relevance of CASP16 Nucleic Acid Predictions as Evaluated by Structure Providers.

Proteins·2025
Same author

Functional relevance of CASP16 nucleic acid predictions as evaluated by structure providers.

bioRxiv : the preprint server for biology·2025
Same author

Molecular basis for the calcium-dependent activation of the ribonuclease EndoU.

Nature communications·2025

Related Experiment Video

Updated: Sep 4, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.9K

Principles and correction of 5'-splice site selection.

Florian Malard1, Cameron D Mackereth1, Sébastien Campagne1

  • 1Inserm U1212, CNRS UMR5320, ARNA Laboratory, University of Bordeaux, Bordeaux Cedex, France.

RNA Biology
|July 22, 2022
PubMed
Summary

This review details how U1 small nuclear ribonucleoproteins (snRNPs) select splice sites in pre-mRNA. It explores factors influencing this selection and therapeutic strategies targeting splice site choice.

Keywords:
5’-splice siteRNA splicingU1 snRNPantisense oligonucleotidessplicing modifiers

More Related Videos

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.6K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.0K

Related Experiment Videos

Last Updated: Sep 4, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.9K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.6K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Eukaryotic gene expression involves splicing, where introns are removed from pre-mRNA.
  • Alternative splicing regulates gene expression by retaining different exons.
  • Spliceosome assembly, a complex process, is crucial for accurate mRNA maturation.

Purpose of the Study:

  • To review the early stage of spliceosome assembly, focusing on U1 snRNP's role in 5'-splice site (5'ss) selection.
  • To discuss factors influencing 5'ss recognition by U1 snRNP.
  • To highlight diseases and therapeutic strategies related to U1 snRNP 5'ss selectivity.

Main Methods:

  • Review of existing literature on pre-mRNA splicing and spliceosome assembly.
  • Analysis of sequence determinants and protein/RNA interactions affecting 5'ss selection.
  • Discussion of disease mechanisms and therapeutic approaches involving U1 snRNP.

Main Results:

  • U1 snRNP plays a critical role in defining the 5'ss during spliceosome assembly.
  • Sequence composition, U1-specific proteins, and U1 snRNA interactions modulate 5'ss selection.
  • Dysregulation of 5'ss selection is linked to various diseases.

Conclusions:

  • Understanding U1 snRNP's 5'ss selectivity is key to comprehending gene regulation and disease.
  • Antisense oligonucleotides and small-molecule splicing switches offer therapeutic potential for modulating splicing.