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Related Experiment Video

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Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell

Cesar A Patino1, Nibir Pathak1,2, Prithvijit Mukherjee1,2

  • 1Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA.

Science Advances
|July 22, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a localized electroporation device (LEPD) with deep learning for rapid optimization of biomolecule delivery into cells. The platform streamlines genetic manipulation for biomanufacturing and therapeutics.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Efficient intracellular delivery of biomolecular cargoes is crucial for biomanufacturing and cell-based therapeutics.
  • Optimizing delivery requires extensive experimental tuning due to multiple influencing factors.

Purpose of the Study:

  • To develop a high-throughput system for rapid optimization of intracellular delivery protocols.
  • To demonstrate the versatility and efficiency of the developed platform for various cell types and delivery applications.

Main Methods:

  • Development of a high-throughput multiwell-format localized electroporation device (LEPD).
  • Integration of deep learning image analysis for rapid assessment of delivery efficiency and cell response.
  • Application of the LEPD platform for single and multi-cargo delivery, including gene knockdown.

Main Results:

  • Successful delivery of biomolecules into diverse adherent and suspension cell types.
  • Demonstration of precise control over multi-cargo delivery, including dosage distribution and ratiometric control.
  • Achieved functional gene knockdown in human induced pluripotent stem cells, with deep learning analysis of protein expression and cell morphology.

Conclusions:

  • The LEPD platform combined with deep learning offers a powerful workflow for combinatorial experiments and rapid optimization of intracellular delivery.
  • This approach significantly accelerates the development of protocols for genetic manipulation in various cell types.
  • The system facilitates advancements in biomanufacturing and the development of cell-based therapeutics.