Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coffee Cascara as a Source of Natural Antimicrobials: Chemical Characterization and Activity Against ESKAPE Pathogens.

Molecules (Basel, Switzerland)·2026
Same author

<i>Plantago major</i> and <i>Plantago lanceolata</i> Exhibit Antioxidant and <i>Borrelia burgdorferi</i> Inhibiting Activities.

International journal of molecular sciences·2024
Same author

Preparation and characterization of lignin-derived carbon aerogels.

Frontiers in chemistry·2024
Same author

Macaronesian Plants as Promising Biopesticides against the Crop Pest <i>Ceratitis capitata</i>.

Plants (Basel, Switzerland)·2023
Same author

Phytochemical Screening and Antioxidant Activity of Selected Estonian <i>Galium</i> Species.

Molecules (Basel, Switzerland)·2023
Same author

The influence of organic solvents on phenylethylamines in capillary zone electrophoresis.

Journal of chromatography. A·2022

Related Experiment Video

Updated: Sep 3, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

14.5K

Extraction of bioactive compounds from Dipsacus fullonum leaves using deep eutectic solvents.

Piret Saar-Reismaa1, Mihkel Koel1, Riin Tarto1

  • 1School of Science, Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.

Journal of Chromatography. A
|July 22, 2022
PubMed
Summary

Deep eutectic solvents (DES) efficiently extracted valuable compounds from Dipsacus fullonum L. leaves, yielding 1.8-2.2 times higher concentrations than conventional methods.

Keywords:
Bioactive compoundsDeep eutectic solventsDipsacus fullonum LExtractionHPLC-DAD-MS

More Related Videos

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.5K
Preparation of Binary and Ternary Deep Eutectic Systems
06:15

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

12.1K

Related Experiment Videos

Last Updated: Sep 3, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

14.5K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.5K
Preparation of Binary and Ternary Deep Eutectic Systems
06:15

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

12.1K

Area of Science:

  • Phytochemistry
  • Green Chemistry
  • Natural Product Extraction

Background:

  • Dipsacus fullonum L. leaves contain bioactive compounds with antioxidant, antimicrobial, anticancer, and anti-Borrelia properties.
  • Traditional organic solvent extraction methods may be less efficient and environmentally impactful.
  • Deep eutectic solvents (DES) offer a promising alternative for selective and efficient extraction of plant bioactives.

Purpose of the Study:

  • To evaluate and optimize deep eutectic solvent (DES) systems for the selective extraction of chlorogenic acid derivatives, flavone glycosides, and iridoid glycosides from Dipsacus fullonum L. leaves.
  • To compare the recovery yields of DES-based extraction with traditional organic solvent methods.
  • To identify the optimal DES composition and extraction parameters for maximizing the recovery of target compounds.

Main Methods:

  • Screening of twelve hydrophilic and hydrophobic DESs, utilizing choline chloride, betaine, menthol as hydrogen bond acceptors (HBA), and various organic acids and glycerol as hydrogen bond donors (HBD).
  • Optimization of the most effective DES composition using Box-Behnken design for selective extraction of three main analyte groups.
  • Quantification and identification of extracted bioactive compounds using High-Performance Liquid Chromatography with Diode-Array Detection and Mass Spectrometry (HPLC-DAD-MS).

Main Results:

  • The optimal DES composition for highest overall recovery was determined to be a molar ratio of choline chloride to lactic acid of 1:2.4, with 35% water and 27 mL of solvent per gram of dry material.
  • The optimized DES extract achieved 1.8 to 2.2 times higher concentrations of target analytes compared to traditional organic solvent extracts.
  • The study demonstrated the selectivity of DES for different groups of bioactive compounds from D. fullonum L. leaves.

Conclusions:

  • Deep eutectic solvents provide a highly efficient and selective method for extracting key bioactive compounds from Dipsacus fullonum L. leaves.
  • Optimized DES extraction significantly enhances the recovery of chlorogenic acid derivatives, flavone glycosides, and iridoid glycosides compared to conventional solvents.
  • This study highlights the potential of DES as a green and effective technology for the sustainable production of valuable phytochemicals from medicinal plants.