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Multilocus, Multiplex Detection of Ganoderma zonatum from Environmental Samples.
Seemanti Chakrabarti1, Andrew L Loyd2, Braham Dhillon1
1Department of Plant Pathology, University of Florida, Fort Lauderdale Research and Education Center, Davie, FL 33314.
Plant Disease
|July 23, 2022
Summary
A new DNA test can detect Ganoderma zonatum, the fungus causing palm butt rot, before visible signs appear. This early detection of Ganoderma zonatum aids in managing palm diseases and preventing tree failure.
Area of Science:
- Plant Pathology
- Mycology
- Molecular Diagnostics
Background:
- Ganoderma butt rot, caused by Ganoderma zonatum, is a destructive palm disease.
- Current diagnostic methods rely on late-stage visual cues like basidiomata (conks).
- Early detection is challenging, hindering effective disease management.
Purpose of the Study:
- To develop a DNA-based molecular diagnostic assay for early detection of Ganoderma zonatum.
- To differentiate G. zonatum from other related Ganoderma species.
- To enable detection before the appearance of characteristic basidiomata.
Main Methods:
- Designed primers targeting single-nucleotide polymorphisms (SNPs) in four genes: ITS, rpb1, rpb2, and tef1-α.
- Utilized a set of three primer pairs for PCR-based detection.
- Tested the assay on environmental samples including sawdust from infected palm trunks and soil.
Main Results:
- The developed assay demonstrated high specificity and sensitivity in detecting G. zonatum.
- Successfully identified G. zonatum in various environmental samples.
- The assay can detect the fungus before the formation of basidiomata.
Conclusions:
- A rapid, DNA-based PCR assay provides a reliable method for early Ganoderma zonatum detection.
- This assay facilitates the identification of inoculum sources and pathogen tracking.
- Early detection is critical for implementing effective palm disease management strategies and mitigating risks.
