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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Sep 3, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

Single-cell sorting based on secreted products for functionally defined cell therapies.

Hiromi Miwa¹, Robert Dimatteo², Joseph de Rutte^1,3

¹Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095 USA.

Microsystems & Nanoengineering

|July 25, 2022

Summary

This summary is machine-generated.

Next-generation microtechnologies are needed to profile and sort single cells based on function, particularly secreted molecules. This innovation will advance the development of potent cellular therapeutics for diverse diseases.

Keywords:

Microfluidics Nanoscience and technology

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Last Updated: Sep 3, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

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Published on: November 10, 2023

Setting a Successful Sorting for Extracellular Vesicle Isolation

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A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Area of Science:

Biotechnology
Cellular Therapeutics
Microfluidics

Background:

Cell therapies show promise for hematological malignancies and other diseases, but their potential is limited by functional variability.
Unlike molecular therapies, cells with identical genetics can exhibit diverse functional properties, hindering therapeutic development.
Current analytical methods lack functional potency-based sorting for cells, exacerbating these differences.

Purpose of the Study:

To highlight the need for advanced single-cell profiling microtechnologies.
To focus on technologies enabling direct evaluation and sorting of single cells based on functional properties, especially secreted molecules.
To address limitations in cellular therapeutic discovery, design, and manufacturing.

Main Methods:

Defining critical processes for single-cell secretion-based profiling: cell partitioning, secretion accumulation/labeling, and signal measurement/sorting.
Summarizing existing academic and commercial technologies for functional single-cell analysis and sorting.
Categorizing technologies into microchamber, microfluidic droplet, and lab-on-a-particle approaches.

Main Results:

Identification of three key technological processes for functional single-cell profiling.
Overview of current microtechnologies applicable to functional single-cell analysis and sorting.
Discussion of industrial applications and unmet needs in cellular therapeutics.

Conclusions:

Next-generation single-cell functional screening technologies are crucial for advancing cellular therapeutics.
These technologies can overcome limitations in potency-based sorting and enable robust cell therapy development.
Realizing these advancements promises to expand the application of cellular therapeutics to a wider range of diseases and patients.