Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

7.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.4K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

19.7K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.7K
Plant Tissue Culture02:57

Plant Tissue Culture

38.4K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
38.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering PE6 prime editors to efficiently insert tags in rice.

Plant biotechnology journal·2024
Same author

Developing a CRISPR/FrCas9 system for core promoter editing in rice.

aBIOTECH·2024
Same author

Editing of upstream regulatory elements advances plant gene silencing.

The New phytologist·2024
Same author

Conditional knockdown of OsMLH1 to improve plant prime editing systems without disturbing fertility in rice.

Genome biology·2024
Same author

A natural variation in OsDSK2a modulates plant growth and salt tolerance through phosphorylation by SnRK1A in rice.

Plant biotechnology journal·2024
Same author

Genome editing with type II-C CRISPR-Cas9 systems from Neisseria meningitidis in rice.

Plant biotechnology journal·2021

Related Experiment Video

Updated: Sep 3, 2025

Scalable Transfection of Maize Mesophyll Protoplasts
08:38

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

3.0K

Development of a highly efficient prime editor 2 system in plants.

Juan Li1, Like Chen2, Jing Liang2

  • 1Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P.R. China.

Genome Biology
|July 25, 2022
PubMed
Summary

We developed an enhanced plant prime editor 2 (enpPE2) system to improve gene editing efficiency in plants. This new system significantly boosts editing frequencies in rice, offering a powerful tool for precise plant genome modification.

Keywords:
EfficiencyEngineeringPrecise editingPrime editingRice

More Related Videos

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

9.8K
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
10:12

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

14.4K

Related Experiment Videos

Last Updated: Sep 3, 2025

Scalable Transfection of Maize Mesophyll Protoplasts
08:38

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

3.0K
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

9.8K
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
10:12

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

14.4K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Prime editing (PE) systems offer precise genome modification but face efficiency limitations in plants.
  • Low editing efficiency restricts the broader application of PE in plant research and crop improvement.

Purpose of the Study:

  • To develop an enhanced plant prime editor 2 (enpPE2) system with significantly improved editing efficiency.
  • To overcome the low efficiency limitations of existing prime editing systems in plant applications.

Main Methods:

  • Updated the prime editor architecture to PEmax.
  • Engineered pegRNA with a structured motif.
  • Expressed the engineered pegRNA under a composite promoter.
  • Implemented these optimizations in the enpPE2 system for plant applications.

Main Results:

  • The enpPE2 system achieved high editing frequencies ranging from 64.58% to 77.08% in T0 rice plants.
  • These frequencies are substantially higher compared to the unmodified pPE2 system.
  • Demonstrated the effectiveness of stacked optimization strategies in enhancing prime editing performance.

Conclusions:

  • The enpPE2 system represents a significant advancement in plant genome editing technology.
  • It provides a robust and powerful tool for precise genetic modifications in plants.
  • The enhanced efficiency facilitates broader applications in plant science and crop development.