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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Ratiometric Fluorescent pH Sensor Based on a Tunable Multivariate Covalent Organic Framework.

Jie-Yu Yue1, Li-Ping Song1, Xiu-Li Ding1

  • 1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, P.R. China.

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|July 26, 2022
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Summary
This summary is machine-generated.

Researchers developed novel covalent organic frameworks (COFs) for reusable ratiometric pH sensing. Tuning component ratios allowed precise control over fluorescent properties and pH detection ranges in acidic conditions.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Ratiometric pH detection is crucial for environmental, medical, and chemical applications.
  • Developing practical, reusable ratiometric pH sensors remains a significant challenge.
  • Covalent organic frameworks (COFs) offer tunable properties for sensing applications.

Purpose of the Study:

  • To synthesize novel three-component covalent organic frameworks (COFs) for ratiometric pH sensing.
  • To investigate the effect of component molar ratios on sensor performance.
  • To evaluate the reusability and applicability of the developed sensors.

Main Methods:

  • Synthesized a series of COFs (COF-COOHX) using Schiff base reactions with varying molar ratios of 2-hydroxybenzene-1,3,5-tricarbaldehyde (HTA), 4,4'-diamino-3,3'-biphenyldicarboxylic acid (DBA), and 5,5'-diamino-2,2'-bipyridine (BPY).
  • Characterized the ratiometric pH sensing performance in acidic conditions.
  • Tuned molar ratios to regulate fluorescent properties, linear pH ranges, and pKa values.

Main Results:

  • Three-component COF-COOHX (X = 33, 50, 67) exhibited selective and repeatable ratiometric pH sensing in acidic media.
  • Two-component COFs (COF-COOH0 and COF-COOH100) lacked ratiometric pH detection ability.
  • The molar ratio of DBA and BPY allowed fine-tuning of fluorescent responses and pH detection parameters.

Conclusions:

  • Novel three-component COFs demonstrate effective ratiometric pH sensing capabilities.
  • The multivariate strategy enables precise control over sensor characteristics.
  • The developed sensors show promise for practical applications in detecting pH in drug solutions and carbonated drinks.