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Updated: Sep 3, 2025

Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding
Published on: July 3, 2020
Generation of Plasmodium falciparum Gametocytes In Vitro with Specific Considerations for Field Isolates
Bismarck Dinko1, Richmond Tackie2, Gisela Henriques3
1Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Ghana. bdinko@uhas.edu.gh.
Abstract:
Reproducible induction of gametocytes of Plasmodium falciparum in vitro is crucial for performing various experimental analyses to understand gametocyte cellular and molecular biology and immunology, and for the evaluation of antigametocidal agents and vaccine development. In this protocol, we present specific procedures for the enrichment, synchronous production and separation of developmental stages of P. falciparum gametocytes from culture-adapted field isolates.

