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Updated: Sep 3, 2025

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

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Published on: February 7, 2022

Resolving icteric interference by a novel photoisomerization device: Bilibox.

Michael A Vera¹, Christopher D Koch¹, Joe M El-Khoury¹

¹Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT, USA.

Clinical Biochemistry

|July 26, 2022

Summary

Icterus interference in lab tests can be resolved using in vitro photoisomerization. A new device, the Bilibox, effectively reduces bilirubin levels, improving creatinine assay accuracy.

Keywords:

Bilibox Bilirubin Creatinine Icteric Interference Photoisomerization

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Published on: February 23, 2017

Area of Science:

Clinical Chemistry
Biochemistry
Analytical Chemistry

Background:

Icterus, caused by elevated blood bilirubin, is a common interference in clinical chemistry testing.
This interference can affect spectral analysis and reagent reactivity, often necessitating sample dilution or rejection.
Current methods for managing icteric samples are limited, impacting laboratory workflow and accuracy.

Purpose of the Study:

To investigate the efficacy of in vitro photoisomerization using a novel device (Bilibox) for resolving icteric interference in laboratory testing.
To assess the impact of photoisomerization on bilirubin levels and creatinine assay accuracy.
To evaluate photoisomerization as an alternative to sample dilution for icteric samples.

Main Methods:

Utilized left-over clinical samples (n=10) with varying icteric levels.
Analyzed baseline and diluted creatinine, total bilirubin (TBIL), direct bilirubin (DBIL), and hemolysis, icterus, and lipemia (HIL) indices.
Treated samples in the Bilibox device for 30 and 60 minutes under optimized conditions (500 nm light at 37°C).
Repeated analyte measurements post-treatment to assess changes.

Main Results:

Bilibox treatment significantly reduced icteric index, TBIL, and DBIL by 33.5-39.9% after 30 minutes and 47.6-63.7% after 60 minutes.
Initial creatinine measurements showed a 5.8% difference between diluted and undiluted samples due to icteric interference.
Post-treatment, the creatinine difference decreased to 0.02% (30 min) and 2.2% (60 min), indicating successful interference resolution (p<0.05).

Conclusions:

In vitro photoisomerization, implemented via the Bilibox device, effectively reduces bilirubin levels in icteric samples.
This method significantly resolves icteric interference in creatinine assays, improving accuracy.
Photoisomerization offers a rapid and viable alternative for managing icteric samples when dilution is not feasible.