Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

129
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
129
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

72
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
72
Special Staining Techniques01:13

Special Staining Techniques

256
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
256
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

87
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
87
Fimbriae, Pili, and Axial Filaments01:28

Fimbriae, Pili, and Axial Filaments

276
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
276
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

2.9K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A <i>Chryseobacterium massiliae</i> pore-forming MACPF domain protein mediates intra and interspecies competition against <i>Bacteroides</i>.

microLife·2026
Same author

Specificities and redundancies in the NEL family of bacterial E3 ubiquitin ligases of <i>Salmonella enterica</i> serovar Typhimurium.

Frontiers in immunology·2024
Same author

Escherichia coli SPFH Membrane Microdomain Proteins HflKC Contribute to Aminoglycoside and Oxidative Stress Tolerance.

Microbiology spectrum·2023
Same author

<i>Salmonella</i> Type III Secretion Effector SrfJ: A Glucosylceramidase Affecting the Lipidome and the Transcriptome of Mammalian Host Cells.

International journal of molecular sciences·2023
Same author

Bacterial capsular polysaccharides with antibiofilm activity share common biophysical and electrokinetic properties.

Nature communications·2023
Same author

Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae.

mBio·2023
Same journal

Correction: Peptine et al. Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Vancomycin-Resistant Enterococci (VRE) in Nosocomial Infections: A Systematic Review of Resistance, Pathogenesis, and Clinical Management. <i>Microorganisms</i> 2026, <i>14</i>, 428.

Microorganisms·2026
Same journal

Torque Teno Virus (TTV) Plasma Load and Immune Reconstitution in People Living with HIV: A Systematic Review.

Microorganisms·2026
Same journal

Optimizing Bacteriophage Screening and Isolation Methods for Microbial Samples Derived from Different Body Sites of Cattle.

Microorganisms·2026
Same journal

Enhanced Biphenyl Degradation by <i>Rhodococcus</i> sp. TG-1 Under Cr(VI) Stress via Modified Biochar Immobilization.

Microorganisms·2026
Same journal

In Vitro Detection of Biologically Active Staphylococcal Enterotoxins Type B and C1 as an Alternative to In Vivo Testing.

Microorganisms·2026
Same journal

Monitoring Hygiene Protocols and Exploring Alternatives to Counteract Resistant Pathogens: A Case Study from Southern Italy on Healthcare-Associated Infection Control.

Microorganisms·2026
See all related articles

Related Experiment Video

Updated: Sep 3, 2025

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

15.8K

Special Issue: Type III Secretion Systems in Human/Animal Pathogenic Bacteria.

Joaquín Bernal-Bayard1, Francisco Ramos-Morales1

  • 1Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.

Microorganisms
|July 27, 2022
PubMed
Summary
This summary is machine-generated.

Type III secretion systems (T3SSs) are crucial for Gram-negative bacteria to interact with host cells. Understanding T3SSs aids in developing novel antimicrobial strategies.

More Related Videos

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.6K
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.2K

Related Experiment Videos

Last Updated: Sep 3, 2025

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

15.8K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.6K
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacteriology

Background:

  • Type III secretion systems (T3SSs) are essential virulence factors in many Gram-negative bacterial pathogens.
  • These complex molecular machines facilitate direct injection of effector proteins into eukaryotic host cells, manipulating host cell functions.

Discussion:

  • T3SSs represent a critical interface for bacterial pathogenesis and host-pathogen interactions.
  • Studying T3SS structure and function provides insights into bacterial virulence mechanisms.

Key Insights:

  • T3SSs are conserved across diverse bacterial species, highlighting their evolutionary importance.
  • Effectors injected by T3SSs can subvert host immunity, promote bacterial invasion, and induce cell death.

Outlook:

  • Targeting T3SSs offers a promising avenue for novel antimicrobial drug development.
  • Further research into T3SS regulation and assembly will elucidate bacterial pathogenesis and inform therapeutic strategies.