Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

186
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
186

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antiviral Activity of the Acyclic Nucleoside Phosphonate Prodrug LAVR-289 against Poxviruses and African Swine Fever Virus.

ACS infectious diseases·2025
Same author

Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus.

PLoS pathogens·2024
Same author

APOBEC3F Is a Mutational Driver of the Human Monkeypox Virus Identified in the 2022 Outbreak.

The Journal of infectious diseases·2023
Same author

Fatal Cowpox Virus Infection in Human Fetus, France, 2017.

Emerging infectious diseases·2021
Same author

Mesenchymal stromal cells confer chemoresistance to myeloid leukemia blasts through Side Population functionality and ABC transporter activation.

Haematologica·2019
Same author

Macrophage-derived oncostatin M contributes to human and mouse neurogenic heterotopic ossifications.

JCI insight·2017

Related Experiment Video

Updated: Sep 3, 2025

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.4K

Efficient Method for Generating Point Mutations in the Vaccinia Virus Genome Using CRISPR/Cas9.

Laetitia Boutin1, Estelle Mosca1, Frédéric Iseni1

  • 1Virology Unit, Institut de Recherche Biomédicale des Armées, 91220 Brétigny-sur-Orge, France.

Viruses
|July 27, 2022
PubMed
Summary

This study introduces a modified CRISPR/Cas9 system for efficiently generating vaccinia virus (VACV) point mutations. This method enables rapid selection of mutant VACV strains for research and therapeutic development.

Keywords:
CRISPR-Cas9PAM sequencehomologous recombinationvaccinia virus

More Related Videos

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.5K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.7K

Related Experiment Videos

Last Updated: Sep 3, 2025

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.4K
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.5K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.7K

Area of Science:

  • Virology
  • Molecular Biology
  • Genetic Engineering

Background:

  • Vaccinia virus (VACV) is a versatile platform for vaccines and cancer therapy.
  • Traditional homologous recombination for VACV genome editing is inefficient.
  • CRISPR/Cas9 enhances recombinant VACV production but struggles with point mutations.

Purpose of the Study:

  • To develop a rapid and efficient method for generating vaccinia virus (VACV) point mutations.
  • To improve targeted mutagenesis for studying VACV-host interactions.

Main Methods:

  • A modified CRISPR/Cas9 system was employed for VACV genome editing.
  • A silent mutation was introduced into the donor gene, targeting the PAM sequence and altering a restriction site.
  • Recombinant VACVs with mutations in the E9L gene were generated and analyzed.

Main Results:

  • The modified CRISPR/Cas9 system facilitated rapid selection of mutant VACV.
  • A proof-of-concept demonstrated successful generation of E9L gene mutants.
  • The introduced silent mutation aided in screening and selection of desired viral mutants.

Conclusions:

  • The developed CRISPR/Cas9 system significantly improves the efficiency of generating point mutations in VACV.
  • This method enables rapid recovery and screening of mutant VACV strains.
  • The approach is valuable for advancing research on VACV and its applications.