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ColourQuant: A High-Throughput Technique to Extract and Quantify Color Phenotypes from Plant Images
Mao Li1, Margaret H Frank2, Zoë Migicovsky3
1Donald Danforth Plant Science Center, St. Louis, MO, USA. mli@danforthcenter.org.
Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2022
Summary
This study introduces ColourQuant, a new automated pipeline for high-throughput plant color phenotyping from images. It enables efficient extraction of color traits crucial for ecological studies and crop breeding.
Area of Science:
- Plant biology
- Agricultural science
- Horticultural science
Background:
- Plant color patterning influences ecological interactions, horticultural breeding, and agricultural performance.
- High-throughput phenotyping of color is vital for understanding plant biology and trait selection in breeding programs.
Purpose of the Study:
- To present ColourQuant, an automated high-throughput pipeline for extracting plant color phenotypes from images.
- To provide researchers with a tool for efficient and accurate color trait analysis.
Main Methods:
- Utilizes mean pixel values for color phenotyping.
- Employs a Gaussian density estimator for CIELAB color analysis.
- Incorporates circular deformation for shape-independent color patterning analysis.
Main Results:
- ColourQuant enables automated extraction of detailed color phenotypes.
- The pipeline offers robust analysis of color traits from digital images.
- Shape-independent analysis of color patterns is achieved through circular deformation.
Conclusions:
- ColourQuant is an effective tool for high-throughput color phenotyping in plants.
- This pipeline facilitates advancements in plant biology research and breeding.
- Automated color analysis supports selection for desirable plant traits.
Keywords:
Color patterningColor phenotypingContinuous color distributionHigh-throughput image acquisitionShape-independent color quantification
