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Related Experiment Video

Updated: Sep 3, 2025

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Single-Cell RNA-Seq Identifies Dynamic Cardiac Transition Program from ADCs Induced by Leukemia Inhibitory Factor.

Jiayi Yao¹, Feiyang Ma^2,3, Li Zhang¹

¹Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.

Stem Cells (Dayton, Ohio)

|July 27, 2022

Summary

This summary is machine-generated.

Leukemia inhibitory factor (LIF) induces cardiac transition in adipose-derived cells (ADCs). This study reveals distinct ADC subpopulations and a time-dependent process involving Nrf2 signaling, leading to cardiomyocyte-like cells.

Keywords:

ADC adipose cardiac transition cell sequencing derived cells leukemia inhibitory factor (LIF)single

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Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

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Last Updated: Sep 3, 2025

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

Area of Science:

Stem cell biology
Regenerative medicine
Cardiovascular research

Background:

Adipose-derived cells (ADCs) show potential for cardiac regeneration due to abundant reserves.
Low efficiency in ADC-cardiac transition is attributed to cellular heterogeneity and unclear mechanisms.
Leukemia inhibitory factor (LIF) is investigated as an inducer for ADC cardiomyogenesis.

Purpose of the Study:

To investigate ADC heterogeneity and cellular kinetics during LIF-induced cardiac transition.
To elucidate the mechanisms underlying ADC-myogenesis and cell fate determination.
To identify factors influencing the efficiency of cardiac regeneration from ADCs.

Main Methods:

Single-cell RNA sequencing of 39,432 ADCs undergoing LIF-induced cardiac transition.
Analysis of cellular kinetics and gene expression patterns.
Pseudotime analysis to map cell trajectories and identify distinct cell populations.

Main Results:

Identified distinct ADC subpopulations with differential responses to LIF during cardiomyogenesis.
Demonstrated that ADC-myogenesis is time-dependent and initiated by transient Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) signaling.
Pseudotime analysis revealed two main cell fate trajectories: activated myofibroblasts and cardiomyocyte-like cells.

Conclusions:

High-resolution insights into ADC heterogeneity and cell fate during cardiac transition.
Understanding the role of Nrf2 signaling in initiating cardiomyogenesis.
Provides a foundation for improving cardiac regeneration strategies using ADCs.