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Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
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Gold nanoparticles (AuNP)-based aptasensor for enteropathogenic Escherichia coli detection
Bugi Ratno Budiarto1, Apon Zaenal Mustopa2, Ratih Asmana Ningrum1
1Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Raya Bogor Street KM.46, Cibinong, Bogor, West Java, 16911, Indonesia.
Molecular Biology Reports
|July 27, 2022
Summary
Researchers developed an aptasensor using gold nanoparticles (AuNPs) to detect Enteropathogenic Escherichia coli (EPEC). This novel biosensor shows promise for rapid, point-of-care diagnosis of diarrheal diseases in infants.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Diagnostics
Background:
- Diarrhea remains a significant cause of infant gastrointestinal illness, particularly in developing nations.
- Current molecular diagnostic techniques for EPEC face challenges in rapid, on-site screening.
- Development of point-of-care diagnostics is crucial for timely intervention.
Purpose of the Study:
- To characterize EPEC-specific DNA aptamers.
- To develop a gold nanoparticle (AuNP)-based aptasensor for EPEC detection.
- To evaluate the aptasensor's performance for point-of-care (POC) diagnosis.
Main Methods:
- Screening of six DNA aptamers against target bacteria using qPCR.
- Measurement of aptamer dissociation (Kd) values via qPCR.
- Colorimetry assay to determine aptasensor specificity and limit of detection (LOD).
Main Results:
- Two DNA aptamers (S8-7 and S10-5) were successfully identified for EPEC detection.
- Aptamer S8-7 showed a Kd of 17.08 nM; S10-5 showed a Kd of 34.14 nM.
- The AuNP-based aptasensor demonstrated high selectivity and specificity for EPEC with an LOD of 10^5 CFU/mL.
Conclusions:
- The developed AuNP-based aptasensor shows potential for EPEC detection.
- Further optimization, including mutagenesis of aptamers, is needed for clinical POC application.
- This technology could improve rapid diagnosis of diarrheal diseases.
