Synergetic Antimicrobial Effect of Silver Nanoparticles Conjugated with Iprodione against Valsa mali

Apple tree canker induced by Valsa mali is a vital disease in apple production around the world, and it highlyimpacts the development of apple industry. It is of great significance to study the inhibition effect of common fungicides and develop new fungistats for comprehensive control of apple tree canker. In this experiment, the inhibition activity of five fungicides, including mancozeb, metalaxyl, iprodione, prochloraz, and difenoconazole along with biosynthesized nanosilver against V. mali, were measured with the mycelium growth rate and agar well diffusion methods. The results showed that iprodione exhibited the best inhibitory effect, the median inhibition concentration (IC₅₀) of iprodione and nanosilver was 0.62 μg.mL^-1 and 45.50 μg.mL^-1, the suppression rate achieved 67.93% at 200 μg.mL^-1 of nanosilver. Moreover, a remarkable additive and synergistic antimicrobial effect was verified when silver nanoparticles were conjugated with iprodione at 9:1, 8:2, 7:3, and 6:4 (v/v), and the toxicity ratio was 1.04, 1.13, 1.01, and 0.98, respectively. It is proven that biosynthesized silver nanoparticles could effectively inhibit Valsa mali, and it is possible to develop and screen silver nanoparticle-based nano pesticides to manage plant diseases synthetically.