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Updated: Sep 3, 2025
Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
Published on: March 11, 2016
Derivative Three-Dimensional Synchronous Fluorescence Analysis of Tear Fluid and Their Processing for the Diagnosis
Katarína Dubayová1, Kristína Krajčíková1, Mária Mareková1
1Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik in Košice, Trieda SNP 1, 040 11 Košice, Slovakia.
Background:
Sensitive and rapid diagnosis of the early stages of glaucoma from tear fluid is a great challenge for researchers.
Methods:
Tear fluid was analyzed using three-dimensional synchronous fluorescence spectroscopy (3D-SFS). Our previously published results briefly describe the main methods which applied the second derivative to a selected synchronous spectrum Δλ = 110 nm in distinguishing between healthy subjects (CTRL) and patients with glaucoma (POAG).
Results:
In this paper, a novel strategy was used to evaluate three-dimensional spectra from the tear fluid database of our patients. A series of synchronous excitation spectra were processed as a front view and presented as a single curve showcasing the overall fluorescence profile of the tear fluid. The second derivative spectrum provides two parameters that can enhance the distinction between CTRL and POAG tear fluid.
Conclusions:
Combining different types of 3D-SFS data can offer interesting and useful diagnostic tools and it can be used as input for machine learning and process automation.
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