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Multi-Platform Detection of MMP-7 in Colorectal Carcinoma
Ivana Večurkovská1,2, Marek Stupák1, Jana Kaťuchová3
1Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovakia.
Matrix metalloproteinase-7 (MMP-7) levels are higher in colorectal cancer (CRC) tissues and serum compared to benign conditions. However, serum MMP-7 may indicate early tumor processes, not late-stage burden, due to complex regulation.
Area of Science:
- Oncology
- Biochemistry
- Molecular Biology
Background:
- Matrix metalloproteinase-7 (MMP-7) is linked to colorectal cancer (CRC) progression.
- Its role in disease staging and as a circulating biomarker requires clarification.
Purpose of the Study:
- To investigate differences in MMP-7 expression and activity between benign and malignant colorectal conditions.
- To assess if serum MMP-7 levels correlate with CRC progression.
Main Methods:
- Utilized Gepia, Protein Atlas, and UALCAN databases for MMP-7 expression.
- Employed ELISA, Western blot, and zymography on patient samples (30 benign, 60 CRC).
Main Results:
- MMP-7 levels were significantly elevated in CRC tissues (4.5x) and serum (3x) compared to benign samples.
- MMP-7 activity was over 4x higher in malignant tissues.
- Observed significant sex-related differences in serum MMP-7 concentrations.
Conclusions:
- Discordance between stable MMP-7 mRNA and declining serum protein suggests complex regulation in advanced CRC.
- Serum MMP-7 may reflect early tumor processes rather than late-stage tumor burden.
- Further research in larger, stage-stratified cohorts is warranted.
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