Self-adaptive virtual microchannel for continuous enrichment and separation of nanoparticles

The transport, enrichment, and purification of nanoparticles are fundamental activities in the fields of biology, chemistry, material science, and medicine. Here, we demonstrate an approach for manipulating nanospecimens in which a virtual channel with a diameter that can be spontaneously self-adjusted from dozens to a few micrometers based on the concentration of samples is formed by acoustic waves and streams that are triggered and stabilized by a gigahertz bulk acoustic resonator and microfluidics, respectively. By combining a specially designed arc-shaped resonator and lateral flow, the in situ enrichment, focusing, displacement, and continuous size-based separation of nanoparticles were achieved, with the ability to capture 30-nm polystyrene nanoparticles and continuously focus 150-nm polystyrene nanoparticles. Furthermore, exosome separation was also demonstrated. This technology overcomes the limitation of continuously manipulating particles under 200 nm and has the potential to be useful for a wide range of applications in chemistry, life sciences, and medicine.