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Ultrafast CEST line scanning as a method to quantify mutarotation kinetics.

Daniel Schache1, Solène Bardin2, Luisa Ciobanu2

  • 1Translational Research Imaging Center, Clinic of Radiology, University of Münster, Münster, Germany.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|July 29, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a fast magnetic resonance technique, chemical exchange saturation transfer (CEST) line scanning (LS), to measure sugar mutarotation kinetics. The method accurately determined sugar isomer ratios and rate constants, validating its effectiveness for biochemical research.

Keywords:
CEST quantificationLine scanningLorentzian fitMutarotation kineticsUltrafast CEST MRI

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biophysics

Background:

  • Mutarotation, the interconversion of sugar isomers, is vital in biochemical pathways.
  • Mutarotase enzymes catalyze these essential reactions in energy metabolism.
  • Accurate measurement of mutarotation kinetics is crucial for understanding these processes.

Purpose of the Study:

  • To implement and validate a fast magnetic resonance technique for measuring sugar mutarotation kinetics.
  • To investigate the isomeric conversion of hexoses and pentoses using this novel method.
  • To compare the results with conventional techniques and established models.

Main Methods:

  • Utilized chemical exchange saturation transfer (CEST) line scanning (LS) on a 9.4 T small animal MRI scanner.
  • Investigated the mutarotation of glucose, galactose, xylose, and arabinose in aqueous solutions.
  • Acquired and analyzed CEST spectra over four hours using established quantification approaches.

Main Results:

  • Achieved ultrafast data acquisition, providing complete CEST spectra in under one minute.
  • Determined equilibrium isomer ratios with 94% accuracy using an adapted chemical exchange (CE) model.
  • Estimated mutarotation rate constants that agreed well with conventional reference values.

Conclusions:

  • CEST LS is a rapid and effective method for measuring mutarotation kinetics.
  • The technique offers high accuracy in determining sugar isomer ratios and reaction rates.
  • This approach provides a valuable tool for biochemical research involving sugar metabolism.