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Updated: Sep 3, 2025

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

LITOS: a versatile LED illumination tool for optogenetic stimulation.

Thomas Christoph Höhener¹, Alex Erich Landolt^1,2, Coralie Dessauges¹

¹Institute of Cell Biology, University of Bern, 3012, Bern, Switzerland.

Scientific Reports

|July 30, 2022

Summary

This summary is machine-generated.

We developed LITOS, an affordable and user-friendly LED illumination tool for optogenetics experiments. This device enables flexible, high-resolution light stimulation without coding, democratizing advanced biological research.

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Last Updated: Sep 3, 2025

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

Published on: October 3, 2017

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Area of Science:

Biotechnology
Molecular Biology
Neuroscience

Background:

Optogenetics enables precise control of biological processes using light.
Existing multi-well optogenetics devices are costly or complex to assemble and operate.
There is a need for accessible, flexible, and user-friendly illumination tools for optogenetics.

Purpose of the Study:

To introduce the LED Illumination Tool for Optogenetic Stimulation (LITOS), an affordable and easy-to-assemble device.
To provide a flexible platform for programming complex light stimulation patterns without coding.
To validate LITOS's performance in controlling cellular signaling pathways.

Main Methods:

LITOS utilizes a printed circuit board controlling a 32x64 LED matrix.
Assembly requires no soldering, and an integrated website provides a user-friendly interface.
The device was validated by measuring MAPK/ERK pathway activity using optogenetic actuators (FGFR1, Raf) under dynamic light stimulation.

Main Results:

LITOS allows for quick assembly and intuitive programming of light stimulation protocols.
The device ensures uniform illumination across cells within various labware formats (multi-well plates, petri dishes, flasks).
Validated experiments demonstrated effective temporal control of cellular signaling pathways.

Conclusions:

LITOS offers an affordable and accessible solution for optogenetic stimulation.
The device democratizes the use of optogenetics by simplifying complex light control.
LITOS facilitates high-throughput optogenetics research with enhanced flexibility and ease of use.