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Published on: June 24, 2018
Human osteoblasts response to different dental implant abutment materials: An in-vitro study
Muataz A Osman1, Rasha A Alamoush2, Evgeny Kushnerev3
1Division of Dentistry, School of Medical Sciences, University of Manchester, Coupland 3 Building, Oxford Road, Manchester M13 9PL, United Kingdom; Periodontology Department, Faculty of Dentistry, The University of Benghazi, Benghazi, Libya; Restorative Department, Faculty of Dentistry, Libyan International Medical University, Benghazi, Libya; Blond McIndoe Laboratories, Division of Cell Matrix Biology & Regenerative Medicine, Faculty of Biology, Medicine & Health, The University of Manchester, 3.106 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom.
Titanium nitride (TiN) and modified polyether ether ketone (m-PEEK) show promising biocompatibility for dental implants. These materials exhibited improved human osteoblast (HOB) proliferation and lower cytotoxicity compared to titanium, cobalt chromium, and zirconia.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Cell Biology
Background:
- Dental implant abutments are crucial for supporting prostheses.
- Material selection influences osseointegration and peri-implant tissue health.
- Evaluating human osteoblast (HOB) response is key to assessing biocompatibility.
Purpose of the Study:
- To compare the biological response of human osteoblasts (HOBs) to five different dental implant abutment materials.
- To assess cell proliferation and cytotoxicity across various time points.
- To identify potentially superior materials for dental implant applications.
Main Methods:
- Investigated titanium (Ti), titanium nitride (TiN), cobalt chromium (CoCr), zirconia (ZrO₂), and modified polyether ether ketone (m-PEEK).
- Assessed HOB proliferation and cytotoxicity using Alamar Blue and LDH assays at days 1, 3, 5, and 10.
- Statistical analysis included two-way ANOVA, one-way ANOVA, and Tukey's post hoc test.
Main Results:
- All materials supported initial HOB proliferation, with ZrO₂ showing lower activity.
- TiN and m-PEEK demonstrated enhanced HOB proliferation and lower cytotoxicity at day 10 compared to Ti, CoCr, and ZrO₂.
- Significant effects of material type and time on proliferation and cytotoxicity were observed (p < 0.0001).
Conclusions:
- Titanium nitride (TiN) and modified polyether ether ketone (m-PEEK) exhibit excellent biocompatibility for dental implant abutments.
- These materials present promising alternatives to traditional abutment materials like titanium, cobalt chromium, and zirconia.
- Further research into TiN and m-PEEK could advance dental implantology.
