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Progress on Multifunction Enzyme-Activated Organic Fluorescent Probes for Bioimaging.

Jie Lian1, Yipeng Wang2,3, Xiaomeng Sun2,3

  • 1College of Criminal Investigation, People's Public Security University of China, Beijing, China.

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|August 1, 2022
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Summary

Enzyme-activated fluorescent probes offer non-invasive bioimaging for cells and bacteria. Multi-enzyme strategies enhance selectivity and real-time imaging, showing promise for intraoperative diagnosis.

Keywords:
AIE fluorophoreenzyme-activated fluorophorehigh selectivitymultifunctionreal-time imaging

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Area of Science:

  • Biomedical Engineering
  • Organic Chemistry
  • Molecular Imaging

Background:

  • Bioimaging is crucial in clinical applications, utilizing both in vivo and in vitro systems.
  • Enzymes play a vital role in biological systems, making enzyme-activated probes valuable tools.
  • Traditional imaging probes have limitations compared to advanced fluorescent techniques.

Purpose of the Study:

  • To review well-designed, multi-functional enzyme-activated organic fluorescent probes.
  • To focus on probe design, fluorescence properties, activation mechanisms, and bioimaging applications.
  • To highlight advancements in selective and real-time bioimaging.

Main Methods:

  • Review of latest developments in enzyme-activated organic fluorescent probes.
  • Analysis of probe design strategies and fluorescence characteristics.
  • Examination of enzyme activation processes and their bioimaging utility.

Main Results:

  • Enzyme-activated fluorophores provide 'off-to-on' fluorescence for sensitive, high-resolution imaging.
  • Organic small molecule probes enable non-invasive, real-time visualization of living cells in small animals.
  • Multi-enzyme activation strategies minimize false positives in complex biological environments.

Conclusions:

  • Enzyme-activated fluorescent probes offer significant advantages over traditional imaging agents.
  • Advanced probe designs facilitate accurate bioimaging of specific cells, tissues, and bacteria.
  • These probes show exciting potential for intraoperative fluorescence imaging and diagnostic tools.