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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jul 14, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging for Spatially Resolved Food Safety Analysis.

Yikang Hou1, Zhuoxi Li1, Jie Lian1

  • 1School of Investigation, People's Public Security University of China, Beijing 100038, China.

Journal of the American Society for Mass Spectrometry
|July 13, 2026
PubMed
Summary

Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) offers spatial insights into food safety, mapping molecule locations and penetration. While not replacing current assays, it complements them by identifying hotspots and providing crucial localization data.

Keywords:
MALDI-MSIauthenticitycontaminantsfood safetymass spectrometry imagingquantitative imagingspatial metabolomics

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Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

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Last Updated: Jul 14, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Area of Science:

  • Food science and analytical chemistry
  • Mass spectrometry imaging applications
  • Food safety and risk assessment

Background:

  • Traditional food safety analysis relies on bulk methods (chromatography, spectroscopy) that average molecular concentrations.
  • These methods cannot determine the spatial distribution of hazardous molecules within complex food matrices.
  • Understanding molecular localization is crucial for assessing exposure hotspots and penetration dynamics.

Purpose of the Study:

  • To review Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) for its role in providing spatial evidence in food safety.
  • To classify existing literature on MALDI-MSI in food safety into direct applications, indirect models, and future opportunities.
  • To evaluate the capabilities and limitations of MALDI-MSI for food safety analysis.

Main Methods:

  • Literature review and classification of studies utilizing MALDI-MSI for food safety applications.
  • Analysis of spatial evidence provided by MALDI-MSI concerning molecule location, penetration, and hotspots.
  • Assessment of reported lateral resolution, sensitivity, and quantitative validity in food and plant MALDI-MSI studies.

Main Results:

  • Direct MALDI-MSI food safety applications are strongest for surface additives and processing contaminants.
  • Evidence for mycotoxins, pesticide residues, allergens, and packaging migrants in food matrices remains sparse.
  • MALDI-MSI offers complementary spatial data (localization, penetration, hotspots) but is analyte- and matrix-dependent.

Conclusions:

  • MALDI-MSI is not a replacement for current regulatory assays (LC-MS/MS, GC-MS) but serves a complementary role in food safety.
  • Its strength lies in providing mechanistic localization and identifying exposure hotspots that bulk methods cannot.
  • Reliable application requires careful control of matrix background, ion suppression, analyte redistribution, and validation.