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Updated: Sep 2, 2025
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
NAPT, an unbiased approach for sequential analysis of the protein N- and C-terminome
Zhongjie Wang1, Wenjuan Yuan1, Lei Zhang1
1Institutes of Biomedical Sciences and Shanghai Cancer Centre, Fudan University, Shanghai, 200032, P. R. China. luhaojie@fudan.edu.cn.
Abstract:
Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the Protein N- and C-Terminome (NAPT) for selective separation of protein termini. Altogether, we identified 2458 protein N-termini and 3056 protein C-termini from the HeLa cell line.
