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Updated: Sep 2, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Cryo-electron Tomography Remote Data Collection and Subtomogram Averaging.

Yuewen Sheng1, Kyle Morris1, Julika Radecke2

  • 1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus.

Journal of Visualized Experiments : Jove
|August 1, 2022
PubMed
Summary
This summary is machine-generated.

Cryo-electron tomography (cryo-ET) advancements enable remote data collection. This guide details cryo-ET setup, remote data acquisition, and structure determination using subtomogram averaging for high-resolution imaging.

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Area of Science:

  • Structural Biology
  • Microscopy Techniques

Background:

  • Cryo-electron tomography (cryo-ET) has advanced significantly with new detectors and software.
  • Remote data collection, accelerated by the pandemic, enhances accessibility to cryo-electron microscopy (cryo-EM).

Purpose of the Study:

  • To provide a comprehensive guide for remote cryo-ET data collection.
  • To detail the workflow for structure determination via subtomogram averaging.

Main Methods:

  • Utilizing Tomo5 software for robust remote cryo-ET data acquisition.
  • Implementing subtomogram averaging with emClarity for structure determination.
  • Demonstrating the protocol with apoferritin as a model sample.

Main Results:

  • Established a streamlined protocol for remote cryo-ET data collection.
  • Successfully determined near-atomic resolution structures using subtomogram averaging.
  • Provided troubleshooting guidance for remote cryo-ET sessions.

Conclusions:

  • Remote cryo-ET data collection is now robust and accessible globally.
  • Advanced software and methods facilitate high-resolution structure determination.
  • This workflow empowers researchers with efficient cryo-ET capabilities.