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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Lifetime-Based Responsive Probes: Design and Applications in Biological Analysis.

Hui Jia1, Yiheng Liu1, Jing-Jing Hu1

  • 1State Key Laboratory of Biogeology and Environmental Geology, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, China.

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Lifetime-based responsive probes offer robust biological detection, unaffected by concentration or depth. This review highlights recent advances in their design and bioanalysis applications.

Keywords:
biological analysisdetectionimaginglifetimeprobe

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biotechnology

Background:

  • Biological analysis and detection are crucial for disease diagnosis, treatment efficacy, prognosis, and tumor recurrence prediction in modern biomedicine.
  • Intensity-based optical probes are widely used but susceptible to environmental factors.
  • Lifetime-based responsive probes offer a more stable and reliable alternative due to their independence from external influences.

Purpose of the Study:

  • To review key advances in lifetime-based responsive probes from 2017-2022.
  • To focus on design strategies and bioanalytical applications of these probes.
  • To discuss current challenges and future directions in the field.

Main Methods:

  • Literature review of scientific publications from 2017-2022.
  • Analysis of design principles for lifetime-based responsive probes.
  • Examination of reported applications in bioanalysis.

Main Results:

  • Significant progress in the design of lifetime-based responsive probes.
  • Demonstrated utility in various bioanalytical applications.
  • Identified key challenges hindering broader adoption and development.

Conclusions:

  • Lifetime-based responsive probes represent a promising technology for bioanalysis.
  • Further research into design and application is warranted.
  • Overcoming current challenges will accelerate their integration into clinical and research settings.