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Updated: Sep 2, 2025
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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
Published on: December 9, 2022
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Long Non-Coding RNA HOTTIP is Elevated in Patients with Sepsis and Promotes Cardiac Dysfunction
Hao Fan1, Han Shao1, Xinyu Gao2
1Department of Critical Care Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
Immunological Investigations
|August 3, 2022
Summary
Long non-coding RNA HOTTIP is elevated in sepsis patients and predicts cardiac dysfunction. Knockdown of HOTTIP reduces LPS-induced cell apoptosis and inflammation, suggesting a therapeutic target for sepsis-related heart problems.
Area of Science:
- Molecular Biology
- Cardiology
- Sepsis Research
Background:
- Sepsis frequently leads to cardiac dysfunction.
- The role of long non-coding RNA (lncRNA) HOTTIP in sepsis-induced cardiac dysfunction is under investigation.
Purpose of the Study:
- To explore the clinical significance of lncRNA HOTTIP in sepsis.
- To investigate the association between HOTTIP and sepsis-induced cardiac dysfunction.
Main Methods:
- Serum HOTTIP levels were measured in 120 sepsis patients using RT-qPCR.
- AC16 cells were treated with lipopolysaccharide (LPS) to assess cell viability and apoptosis.
Main Results:
- Elevated serum HOTTIP levels were observed in sepsis patients and correlated with procalcitonin (PCT).
- Serum HOTTIP demonstrated high diagnostic accuracy (AUC=0.927) for sepsis and predicted cardiac dysfunction development.
- HOTTIP knockdown in vitro reduced LPS-induced apoptosis and IL-6 release.
Conclusions:
- LncRNA HOTTIP is a potential biomarker for sepsis and cardiac dysfunction.
- HOTTIP may play a role in LPS-induced myocardial apoptosis and inflammation, suggesting it as a therapeutic target.
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