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Updated: Jul 19, 2026

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
Published on: November 15, 2011
A highly automated apparatus for ultra-fast laser ablation studies
Marnix Vreugdenhil1, Dries van Oosten1
1Debye Institute for Nanomaterials Science and Center for Extreme Matter and Emergent Phenomena, Utrecht University, Princetonplein 5, 3584 CC Utrecht, The Netherlands.
Abstract:
We present a novel experimental apparatus that can be used for extensive systematic studies of (single- and multi-shot) ultra-short laser pulse ablation. It is fully automated and generates a large number of ablation sites in a short time on a small sample surface area. For each site, the apparatus takes four in situ images: an image of the incident ablation beam (to determine pulse energy), a white light reference image of the pristine sample site, an image of the reflected ablation spot, and a white light image of the ablated sample site. The setup can perform ablation experiments as a function of many parameters, including pulse energy, pulse duration, number of pulses, time between pulses, and focus size. As a proof of concept, we present example results on single-shot ablation off crystalline silicon. Using only data acquired in situ in the presented setup, we determine the single-shot ablation threshold as a function of pulse duration and verify the threshold value using optical interferometric profilometry. The values we found agree well with literature values.

