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Surface modified hexagonal upconversion nanoparticles for the development of competitive assay for biodetection.

Padmaja Parameswaran Nampi1, Alexander Vakurov2, Sikha Saha3

  • 1School of Chemical and Process Engineering, Faculty of Engineering and Physical Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom; School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), Faculty of Medicine and Health, University of Leeds, Leeds LS2 9JT, United Kingdom.

Biomaterials Advances
|August 5, 2022
PubMed
Summary

Researchers developed up-conversion nanoparticles (UCNPs) for detecting the antibiotic ofloxacin. These modified nanoparticles form the basis of a sensitive and rapid photoluminescent nanobiosensor.

Keywords:
Competitive assayDetectionOfloxacinPhotoluminescenceUp-conversion nanoparticles

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Up-conversion nanoparticles (UCNPs) offer unique optical properties for sensing applications.
  • Sodium yttrium fluoride UCNPs doped with ytterbium (Yb3+) and erbium (Er3+) ions exhibit strong green luminescence.
  • Surface modification is crucial for integrating UCNPs into biosensing platforms.

Purpose of the Study:

  • To synthesize and characterize uniform β-NaYF4/Yb3+/Er3+ UCNPs.
  • To develop a stable, aqueous-compatible UCNP platform for biomolecule detection.
  • To create a photoluminescent nanobiosensor for the detection and quantification of ofloxacin.

Main Methods:

  • Solvothermal synthesis of β-NaYF4/Yb3+/Er3+ UCNPs with controlled size and hexagonal phase.
  • Silica coating to enhance aqueous stability.
  • Surface functionalization with maleimide-polyethylene glycol-silane (mal-PEG-silane) for antibody conjugation.
  • Competitive immunoassay utilizing antibody-conjugated UCNPs for ofloxacin detection.

Main Results:

  • Uniform ~50 nm hexagonal UCNPs with strong green up-conversion luminescence were synthesized.
  • Silica coating rendered the UCNPs stable in aqueous media.
  • Maleimide-functionalized UCNPs were successfully conjugated to antibodies.
  • A sensitive competitive assay detected ofloxacin down to ~10 nM.

Conclusions:

  • The developed UCNP-based nanobiosensor is effective for detecting ofloxacin.
  • The assay demonstrates high sensitivity and speed, suitable for veterinary antibiotic monitoring.
  • This platform holds promise for developing sensors for various biomolecules.