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Related Concept Videos

Role of Septins01:02

Role of Septins

1.8K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
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Septins01:19

Septins

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Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
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The Sarcomere01:08

The Sarcomere

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A sarcomere is a microscopic segment repeating in a myofibril. The sarcomere fundamentally consists of two main myofilaments: thick filaments called myosin and thin filaments called actin. These filaments interact by sliding past each other in response to stimulus. In addition to myosin and actin, several other proteins, such as tropomyosin, troponin, titin, nebulin, myomesin, α-actinin, and dystrophin, play crucial roles in regulating, structuring, and functioning of the sarcomere.
Each...
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Cytoskeletal Accessory Proteins01:13

Cytoskeletal Accessory Proteins

3.2K
The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...
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Structural Protein Function01:56

Structural Protein Function

28.2K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
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Microscopic Anatomy of Skeletal Muscles01:13

Microscopic Anatomy of Skeletal Muscles

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Skeletal muscle cells, also called muscle fibers, are distinctly elongated, multi-nucleated, slender biological units. They are packed with specialized structures designed to facilitate their primary function, which is contraction.
The muscle sarcolemma is a plasma membrane enclosing each muscle cell that conducts electrical signals called action potentials. The sarcolemma extends into the cell to form T-tubules, ensuring the neural impulses are uniformly distributed across the entire muscle...
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Correction: Singlár et al. Revealing the Specific Contributions of Mitochondrial CB<sub>1</sub> Receptors to the Overall Function of Skeletal Muscle in Mice. <i>Cells</i> 2025, <i>14</i>, 1517.

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Related Experiment Video

Updated: Sep 2, 2025

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

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Septin7 is indispensable for proper skeletal muscle architecture and function.

Mónika Gönczi1, Zsolt Ráduly1,2, László Szabó1,2

  • 1Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Elife
|August 5, 2022
PubMed
Summary

Septin7 is crucial for skeletal muscle development and function. Its absence causes deformities, reduced muscle force, and impaired cell division and differentiation, highlighting its role in muscle health and regeneration.

Keywords:
cell biologycytoskeletondevelopmenthumanmitochondriamouseregenerationseptinsskeletal muscle

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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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Area of Science:

  • Cell Biology
  • Muscle Physiology

Background:

  • Septins form the fourth component of the cytoskeleton.
  • Septin7 is vital for higher-order structure formation.
  • Septin7's role in skeletal muscle remains largely unexplored.

Purpose of the Study:

  • To investigate the function and expression of Septin7 in skeletal muscle.
  • To elucidate the role of Septin7 in muscle development, differentiation, and regeneration.

Main Methods:

  • Septin7 conditional knockdown (KD) mouse model.
  • C2C12 myoblast cell line.
  • Enzymatically isolated adult muscle fibers.

Main Results:

  • Septin7 KD mice exhibited skeletal deformities, reduced muscle force, and disorganized mitochondria.
  • Septin7 knockout in C2C12 cells led to a complete loss of cell division.
  • Septin7 KD impaired myotube differentiation, with increased expression post-injury.

Conclusions:

  • Septin7 is essential for skeletal muscle development, differentiation, and regeneration.
  • Septin7 plays a critical role in maintaining muscle structure and function.
  • Further research into Septin7's role in muscle injury and repair is warranted.