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What is Genetic Engineering?

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Related Experiment Video

Updated: Sep 2, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Genome Engineering for Next-Generation Cellular Immunotherapies.

Jonathan J Park^1,2,3,4,5, Kyoung A V Lee^1,2,3,6, Stanley Z Lam^1,2,3

¹Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, United States.

|August 5, 2022

Summary

This summary is machine-generated.

Genome engineering advances CAR-T, TCR-T, and NK cell therapies for cancer by overcoming challenges like antigen escape and improving allogeneic treatments. These sophisticated genetic tools enhance cell potency and control for better cancer immunotherapy outcomes.

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Last Updated: Sep 2, 2025

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

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Published on: May 9, 2025

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Published on: November 3, 2020

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

Area of Science:

Immunology
Genetics
Biotechnology

Background:

Cellular immunotherapies like CAR-T, TCR-T, and NK cell therapies have shown success in cancer treatment.
Challenges persist, including antigen escape, tumor microenvironment immunosuppression, toxicities, and off-tumor effects.

Purpose of the Study:

To explore how genome engineering strategies can overcome current limitations in cellular immunotherapies.
To highlight the role of CRISPR-Cas and synthetic biology in advancing cancer treatment.

Main Methods:

Utilizing multiplexed CRISPR-Cas and synthetic biology for genetic modifications.
Targeting genes for enhanced persistence, immunosuppression resistance, and allogeneic compatibility (e.g., TCR, MHC-I knockout).
Exploring genome engineering for CAR macrophages and CAR-NK cells.

Main Results:

Genome engineering enables enhanced potency and modular control of cell therapies.
Strategies address antigen escape, immunosuppression, and host-graft interactions in allogeneic settings.
Development of advanced cellular immunotherapies, including CAR macrophages and CAR-NK cells.

Conclusions:

Genome engineering is crucial for overcoming challenges and improving the efficacy of cellular immunotherapies.
CRISPR-Cas and synthetic biology offer powerful tools for next-generation cancer treatments.
Future research may leverage unbiased CRISPR screening for novel immunotherapy target discovery.