Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial distribution of the proteome in the human body and in cancers.

Nature·2026
Same author

Allelic variation in UVR8 modulates thermotolerance-yield tradeoffs in plants.

Cell research·2026
Same author

DDA-BERT: end-to-end training for data-dependent acquisition mass spectrometry-based proteomics.

Nature communications·2026
Same author

Deeper is not always better in plasma proteomics.

Nature biotechnology·2026
Same author

Longitudinal multimorbidity trajectories shape personalized glycaemic patterns.

Nature metabolism·2026
Same author

The 2025 Westlake Autumn Symposium for Al Proteomics and Virtual Cell.

Genomics, proteomics & bioinformatics·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
Same journal

Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

Nature protocols·2026
Same journal

Facilitating structure-based drug discovery with an artificial intelligence-driven virtual screening platform.

Nature protocols·2026
Same journal

Yeast nuclei-mediated precise delivery of synthetic megabase-scale human DNA into mammalian embryos.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Sep 2, 2025

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

12.6K

High-throughput proteomic sample preparation using pressure cycling technology.

Xue Cai1,2, Zhangzhi Xue1,2, Chunlong Wu3

  • 1Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China.

Nature Protocols
|August 5, 2022
PubMed
Summary
This summary is machine-generated.

Pressure cycling technology (PCT) offers a rapid, robust method for preparing biopsy tissues for clinical proteomics. This technique efficiently lyses and digests proteins from various sample types in approximately 3 hours.

More Related Videos

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

10.1K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

804

Related Experiment Videos

Last Updated: Sep 2, 2025

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

12.6K
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

10.1K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

804

Area of Science:

  • Biochemistry
  • Proteomics
  • Biotechnology

Background:

  • High-throughput tissue sample preparation is crucial for clinical proteomics.
  • Existing methods for lysis and proteolytic digestion of biopsy tissues are often time-consuming and inefficient.
  • A bottleneck exists in processing small tissue specimens for proteomic analysis.

Purpose of the Study:

  • To describe a detailed protocol for pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of biopsy tissues.
  • To establish a reproducible, robust, and efficient method for protein extraction and digestion.
  • To demonstrate the versatility of the method across different sample types and conditions.

Main Methods:

  • Utilized pressure cycling technology (PCT) with specialized PCT-MicroTubes and a Barocycler.
  • Applied oscillating pressure (1 to ~3,000 atm) for tissue lysis and proteolytic digestion.
  • Optimized pressure cycling parameters for efficient protein extraction and peptide generation from various tissues.

Main Results:

  • Developed an efficient protocol for preparing fresh frozen and formalin-fixed paraffin-embedded (FFPE) biopsy tissues.
  • Demonstrated successful application to diverse biological samples including cells, feces, and tear strips.
  • Processed 16 samples in a single batch within approximately 3 hours, yielding peptides for mass spectrometry-based proteomics.
  • Validated the protocol using mouse kidney tissue and eight human tumor types.

Conclusions:

  • PCT-assisted sample preparation provides a rapid and effective solution for clinical proteomics.
  • The protocol is versatile, applicable to various sample types and conditions, including FFPE tissues.
  • This method enhances throughput and reproducibility for mass spectrometry-based proteomic analyses.