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Updated: Sep 2, 2025
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Phosphoproteome profiling of mouse liver during normal aging.
Jiang-Feng Liu1, Yue Wu1,2, Ye-Hong Yang1
1State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China.
Aging in mice liver shows altered protein phosphorylation, impacting fatty acid metabolism and cell signaling pathways. This phosphoproteome analysis reveals key changes linked to aging-associated fatty liver, offering insights for anti-aging interventions.
Area of Science:
- Gerontology
- Molecular Biology
- Biochemistry
Background:
- Aging is a natural process causing functional decline.
- Omics studies aid in understanding aging mechanisms.
- Older mice often exhibit obesity and fatty liver disease.
Purpose of the Study:
- To investigate age-related changes in liver phosphoproteome.
- To identify key phosphorylation pathways affected by aging.
- To correlate phosphoproteomic data with aging-associated liver conditions.
Main Methods:
- Mass spectrometry-based phosphoproteomics applied to mouse liver.
- Quantitative analysis using MaxQuant and principal component analysis (PCA).
- Kinase prediction, kinase-substrate interaction, and KEGG pathway analysis.
Main Results:
- 5,685 phosphosites across 2,335 proteins quantified.
- PCA distinguished young from old mice based on phosphoproteome and transcriptome.
- Increased phosphorylation in fatty acid biosynthesis and secretion pathways; decreased in EGFR signaling and Ctnnb1.
Conclusions:
- A phosphorylation network associated with mouse liver aging was established.
- Phosphoproteome data provides deeper insights into aging-related liver changes than transcriptomics.
- Findings may guide the development of novel anti-aging strategies.
