A microspectroscopic analysis of ligand binding in single erythrocyte

The kinetics of ligand uptake by erythrocytes has been analyzed by single cell microspectroscopy measurements carried out in parallel with mathematical simulations. The main experimental feature is the linear shape of the ligand binding progress curves, whose slope is dependent on the free ligand concentration in the speciman. From the computer simulation a predominant role has been shown to be played by the ligand diffusion from the extracellular space.