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Electrochemical biosensor based on three components random conjugated polymer with fullerene (C60)
Sevinc Kurbanoglu1, Sevki Can Cevher2, Levent Toppare3
1Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06560 Ankara, Turkey.
Bioelectrochemistry (Amsterdam, Netherlands)
|August 7, 2022
Summary
A novel electrochemical biosensor using a conjugated polymer and fullerene was developed for detecting indomethacin (INDO) by monitoring Tyrosinase (Tyr) enzyme inhibition. This sensitive biosensor offers a fast response for indomethacin detection.
Area of Science:
- Electrochemistry
- Materials Science
- Biochemistry
Background:
- Tyrosinase (Tyr) enzyme plays a crucial role in various biological processes and is a target for drug development.
- Developing sensitive and rapid biosensors for drug active compounds is essential for pharmaceutical research and quality control.
- Indomethacin (INDO) is a nonsteroidal anti-inflammatory drug whose mechanism of action can involve enzyme inhibition.
Purpose of the Study:
- To develop a novel conjugated polymer and fullerene-based electrochemical biosensor for Tyrosinase (Tyr) enzyme inhibition.
- To utilize this biosensor for the detection of the active drug compound indomethacin (INDO).
- To characterize the performance and sensitivity of the developed catechol biosensor.
Main Methods:
- Fabrication of a modified electrode using a conjugated polymer (poly[BDT-alt-(TP;BO)]) and fullerene.
- Electrochemical characterization using cyclic voltammetry and electrochemical impedance spectroscopy.
- Surface morphology analysis using scanning electron microscopy and atomic force microscopy.
- Chronoamperometric measurements for catechol detection and Tyr inhibition studies.
Main Results:
- The modified electrode exhibited excellent electrochemical and surface properties.
- The biosensor achieved sensitive detection of catechol within a range of 0.5 to 62.5 µM, with a limit of detection of 0.11 µM.
- Indomethacin (INDO) was found to inhibit Tyr enzyme activity with a mixed-type inhibition characteristic and an IC50 value of 15.11 µM.
Conclusions:
- A highly sensitive and fast-response catechol biosensor was successfully developed using a unique conjugated polymer and fullerene architecture.
- The developed biosensor provides an effective platform for monitoring Tyrosinase (Tyr) enzyme inhibition.
- The study demonstrates the potential of this biosensor for the detection and kinetic analysis of indomethacin (INDO) drug active compound.
