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Reduction of H3K27cr Modification During DNA Damage in Colon Cancer

Meijian Liao1, Weiwei Chu2, Xiaolin Sun1

  • 1Department of Pathology, Xuzhou Medical University, Xuzhou, China.

Frontiers in Oncology
|August 8, 2022
PubMed

Insights

Histone crotonylation is linked to DNA damage activity in colon cancer. Its levels decrease with DNA damage treatments, and sirtuin 6 regulates this modification, offering new insights into cancer mechanisms.

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Molecular Oncology

Background:

  • DNA damage is crucial in colon cancer development.
  • Histone crotonylation, a novel epigenetic modification, enhances gene expression.
  • The role of histone crotonylation in DNA damage and colon cancer is unexplored.

Purpose of the Study:

  • To investigate the association between histone crotonylation and DNA damage in colon cancer.
  • To elucidate the molecular mechanisms linking histone crotonylation to DNA damage in this context.

Main Methods:

  • Bioinformatics analysis of gene promoters.
  • Western blotting to assess histone modification levels.
  • Investigation of sirtuin 6's role in regulating histone crotonylation.

Main Results:

  • Genes associated with DNA damage activity in colon cancer patients showed promoter occupancy by histone crotonylation.
  • Histone crotonylation at Lys27 of histone H3 (H3K27cr) decreased upon treatment with DNA-damaging agents (camptothecin, etoposide).
  • Sirtuin 6 was identified as a regulator of cellular H3K27cr levels.

Conclusions:

  • Histone crotonylation is implicated in DNA damage pathways within colon cancer.
  • The study reveals a novel regulatory role for sirtuin 6 in H3K27cr levels.
  • Findings provide a new perspective on the interplay between histone crotonylation and DNA damage in colon cancer.
Keywords:
DNA damageH3K27crSIRT6etoposidehistone crotonylation

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