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Updated: Sep 2, 2025

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
A rod-like melem with high fluorescence quantum yield for sensitive detection of reduced glutathione
Xieyin Li1, Yuqian Zhang1, Mian Wei1
1Hebei Provincial Key Laboratory of Inorganic Nonmetallic Materials, College of Materials Science and Engineering, North China University of Science and Technology, Tangshan 063210, Hebei, China.
Abstract:
A rod-like melem with high fluorescence quantum yield of 71.3 % was prepared in this work to enhance the chemiluminescence (CL) intensity of Na2SO3-Ce (Ⅳ) system. The results showed that the CL intensity of Na2SO3-Ce (Ⅳ) system could be increased by 350 times based on chemiluminescence resonance energy transfer (CRET) mechanism. Furthermore, a CL sensor based on Na2SO3-Ce (Ⅳ)-melem system was designed to detect reduced glutathione (G-SH). It was indicated that the CL sensor exhibited excellent G-SH detection performance with a detection limit of 0.065 nM and a linear range from 0.32 to 650 μM. This study applied melem for CL detection and provided a new way for the detection of G-SH.

