Enhanced electrochemiluminescence of dual-defect graphite carbon nitride for ultrasensitive detection of CEA

Herein, a dual-defective graphite carbon nitride (DDCN) was prepared by polymerization under N₂ atmosphere combined with oxidation treatment. The luminous intensity of dual-defect graphite phase carbon nitride based on defect state luminescence is significantly improved compared to CN-air. On this basis, a biosensor for CEA detection was constructed based on specific immunobinding of antigen-antibody. It is noted that the biosensor exhibits a wide linear range of 1 × 10^-5 ∼ 1 × 10² ng•mL^-1, a low detection limit of 3.3 × 10^-4 pg•mL^-1, a recovery of 94 %∼105 % and RSD less than 4.41 %. In addition, there was no significant difference to the clinical results, indicating that this work has good clinical application prospects.