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RNA In Situ Hybridization of Whole-Mount Mosquito Olfactory Tissues

Margaret Herre1,2

  • 1The Laboratory of Neurogenetics and Behavior, The Rockefeller University, New York, New York 10065, USA mherre@gmail.com.

Cold Spring Harbor Protocols
|August 8, 2022
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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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