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Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...

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Updated: Sep 2, 2025

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

Measuring anion binding at biomembrane interfaces.

Xin Wu¹, Patrick Wang¹, William Lewis¹

¹School of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia.

Nature Communications

|August 8, 2022

Summary

This summary is machine-generated.

Anion binding in lipid bilayers differs from solution behavior. Charge-diffuse anions show surprising affinity due to polarizability and membrane interaction, unlike charge-dense anions.

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Last Updated: Sep 2, 2025

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

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Published on: November 29, 2014

Area of Science:

Supramolecular Chemistry
Biophysical Chemistry
Membrane Science

Background:

Quantifying anion binding within lipid bilayers is difficult.
Understanding anion interactions at biomembrane interfaces is crucial for biological systems.

Purpose of the Study:

To investigate anion binding mechanisms within lipid bilayers.
To identify factors governing anion recognition at membrane interfaces.

Main Methods:

Development of a fluorescent macrocycle with high sulfate affinity.
Measurement of anion binding affinities in lipid bilayers versus solution (dimethyl sulfoxide).

Main Results:

Anion binding determinants in lipid bilayers differ significantly from solution.
Charge-dense anions (H2PO4-, Cl-) showed low binding in lipids.
Charge-diffuse anions (ClO4-, I-) exhibited high binding affinity in lipid bilayers.
Binding affinity correlates with anion polarizability and complex penetration depth.

Conclusions:

A new principle for lipid bilayer anion binding is revealed, favoring charge-diffuse anions.
Insights guide the design of molecular systems for biomembrane interfaces.
Enhances understanding of biological anion selectivity.