Microfabricated Liquid Junction Capillary Electrophoresis-Mass Spectrometry Interface
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Updated: Sep 2, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Jana Krenkova1, Frantisek Foret2
1Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic. krenkova@iach.cz.
Abstract:
Coupling of capillary electrophoresis (CE) with mass spectrometry (MS) represents a powerful combination for performing rapid, efficient, and sensitive analysis of a variety of compounds. Here we describe a construction, operation, and application of a microfabricated liquid junction CE-MS interface. The interface is designed as a microfabricated unit with an integrated liquid junction and electrospray tip made from polyimide, which is positioned in a plastic connection block securing the separation CE capillary and attachable to the CE instrument. The application was demonstrated by CE-MS analysis of dextran oligomers labeled by (2-aminoethyl)trimethylammonium (AETMA) salt.
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