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Related Experiment Videos

Cefoxitin interferes with the "Clini-Skreen" column method for urinary 17-hydroxycorticosteroids.

M H Kroll, A J Jackson, R J Elin

    Clinical Chemistry
    |July 1, 1987
    PubMed
    Summary

    Cefoxitin falsely elevates urinary 17-hydroxycorticosteroid measurements by creating a similar light-absorbing compound. This interference occurs because cefoxitin reacts with phenylhydrazine, forming a chromophore that mimics the hormone's spectral properties.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Pharmacology

    Background:

    • Accurate measurement of urinary 17-hydroxycorticosteroids is crucial for diagnosing adrenal disorders.
    • Cefoxitin is an antibiotic known to interfere with certain laboratory assays.
    • Previous observations indicated elevated hormone levels in patients taking cefoxitin.

    Purpose of the Study:

    • To elucidate the mechanism by which cefoxitin interferes with urinary 17-hydroxycorticosteroid determination.
    • To compare the reaction kinetics and spectral properties of cefoxitin with relevant steroids and phenylhydrazine.

    Main Methods:

    • Spectrophotometric analysis of reactions between cefoxitin, cortisol, cortisone, 11-deoxycortisol, and phenylhydrazine.
    • Determination of molar absorptivities, equilibrium constants, and rate constants for these reactions.

    Related Experiment Videos

  • Comparison of absorption spectra of reaction products with other cephalosporins and penicillin.

    • Main Results:

    • Cefoxitin, cortisol, cortisone, and 11-deoxycortisol form reaction products with phenylhydrazine that exhibit superimposable spectra and an absorption maximum at 410 nm.
    • While exhibiting similar spectral characteristics, cefoxitin reacts 35-fold slower and has a ninefold lower equilibrium constant compared to the natural steroids.
    • Other tested antibiotics did not produce a chromophore with an absorption maximum at 410 nm.

    Conclusions:

    • Cefoxitin interferes with urinary 17-hydroxycorticosteroid assays by forming a phenylhydrazone chromophore with a similar absorbance maximum (410 nm) to cortisol.
    • The slower reaction rate and lower equilibrium constant of cefoxitin explain its interference, leading to falsely elevated apparent hormone concentrations.