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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
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Fluorescence and Phosphorescence: Instrumentation01:25

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

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Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
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IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
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Phosphorescent Ir(III) Complexes for Biolabeling and Biosensing.

Byung Hak Jhun1,2, Dayoon Song1,3, Soo Young Park4

  • 1Division of Chemical Engineering and Materials Science, Ewha Womans University, Seoul, 03760, Republic of Korea.

Topics in Current Chemistry (Cham)
|August 10, 2022
PubMed
Summary
This summary is machine-generated.

Phosphorescent iridium(III) complexes are versatile tools for bioimaging. Their tunable properties enable visualization of cellular structures and sensing of various analytes, highlighting their potential in molecular imaging.

Keywords:
BiolabelsBiosensorsIr(III) complexPhosphorescence

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Area of Science:

  • Inorganic Chemistry
  • Photochemistry
  • Bioimaging

Background:

  • Cyclometalated iridium(III) complexes display robust phosphorescence at room temperature.
  • Their synthetic tunability allows precise control over charge, lipophilicity, and emission color.
  • These characteristics make them highly suitable for luminescent bioimaging.

Purpose of the Study:

  • To review recent advancements in phosphorescent biolabels and sensors utilizing iridium(III) complexes.
  • To highlight the broad utility and potential of these complexes in molecular imaging.

Main Methods:

  • Introduction to the synthesis and photophysical principles of cyclometalated iridium(III) complexes.
  • Summarization of applications in cellular and in vivo imaging.
  • Discussion of sensor development through chemical modification.

Main Results:

  • Iridium(III) complexes effectively label intracellular organelles like mitochondria, lysosomes, and cell membranes.
  • They enable visualization of complex biological structures such as tumor spheroids and parasites.
  • Modified complexes demonstrate significant sensing capabilities for diverse analytes including temperature, pH, and reactive oxygen species.

Conclusions:

  • Phosphorescent iridium(III) complexes offer a versatile platform for advanced bioimaging.
  • Their adaptability facilitates the development of novel biolabels and sophisticated sensors.
  • These complexes hold substantial promise for future applications in molecular diagnostics and therapeutics.