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Updated: Sep 1, 2025

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Published on: September 12, 2019
Fiber Diffraction and Small-Angle Scattering for Structural Investigation of Bacterial Amyloids
Tatsuhito Matsuo1,2,3, Judith Peters4,5,6
1Univ. Grenoble Alpes, CNRS, LiPhy, Grenoble, France. matsuo@ill.fr.
Abstract:
X-ray/neutron fiber diffraction and small-angle X-ray/neutron scattering are widely used to investigate the molecular structure of fibrous proteins, including amyloid fibrils. However, there is sometimes confusion between these two techniques despite the fact that sample conditions and the content of the information obtained are not the same. In this brief chapter, we present the differences in sample conditions between these two methods, and their effects on experimentally obtained diffraction or scattering patterns, emphasizing the degree of disorder in the samples.
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