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Updated: Sep 1, 2025

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Assessing cytochrome P450 function using genetically engineered mouse models.

Sarrah L Hannon¹, Xinxin Ding¹

¹Department of Pharmacology and Toxicology, Ken R. Coit College of Pharmacy, The University of Arizona, Tucson, AZ, United States.

Advances in Pharmacology (San Diego, Calif.)

|August 11, 2022

Summary

This summary is machine-generated.

Genetically engineered mouse models, including knockout and humanized models, are crucial for understanding cytochrome P450 (CYP) enzyme functions in drug metabolism and disease. These models advance research into xenobiotic interactions and personalized medicine.

Keywords:

Carcinogenesis Cytochrome P450 Drug disposition Drug toxicity Humanized Knockout Mice Transgenic

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Last Updated: Sep 1, 2025

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Published on: January 7, 2019

Area of Science:

Pharmacology
Toxicology
Genetics

Background:

Genetically engineered mouse models (GEMMs) have revolutionized scientific research by enabling gene manipulation.
Cytochrome P450 (CYP) enzymes play critical roles in metabolizing drugs, xenobiotics, and endogenous compounds.
Understanding CYP functions is vital for drug development, toxicology, and disease susceptibility.

Purpose of the Study:

To provide a comprehensive list of CYP-targeted mouse models (knockout, transgenic, humanized) within the CYP1-4 families.
To highlight the utility of these models in assessing in vivo xenobiotic metabolism, bioactivation, and toxicity.
To encourage broader adoption of GEMMs for advancing drug metabolism and toxicology research.

Main Methods:

Compilation of existing literature on CYP-knockout, human CYP-transgenic, and CYP-humanized mouse models.
Categorization of models based on targeted CYP gene families (CYP1-4).
Review of studies utilizing these models to investigate xenobiotic metabolism and toxicity.

Main Results:

Detailed overview of available CYP-engineered mouse models.
Demonstration of model utility in evaluating drug metabolism, carcinogen bioactivation, and toxicity.
Identification of specific CYP enzymes and their roles in various biological processes.

Conclusions:

Engineered mouse models are indispensable tools for in vivo drug metabolism and toxicology studies.
These models enhance understanding of CYP enzyme functions, aiding in the development of safer therapeutics.
Increased utilization of GEMMs will improve prediction of adverse drug reactions and environmental disease susceptibility.